Studies on the active center of human plasmin. Partial amino acid sequence of a peptide containing the active center serine residue.
نویسندگان
چکیده
Tryptic digestion of the diisopropyl phosphoryl-32P-Scarboxymethyl light chain derivative of human plasmin followed by gel filtration on Sephadex G-75 and G-25 and chromatography on Dowex SOW-X2 has resulted in the isolation of three radioactive peptides. These three peptides, having 31,33, and 34 residues, are all derived from the same segment of the plasmin molecule. Structural determinations have yielded the partial amino acid sequence, Val-Glx-(Ser-Thr, Glx)-Leu-(Gly, Ala)-His-Leu-Ala-CysAsn-(Thr, Gly, Gly)-Ser-Cys-Gln-Gly-Asp-Ser(diisopropy1 phosphoryl-32P)-Gly-Gly-Pro-Leu-Val-Cys-Phe-Glu-LysAsp-Lys-Tyr.
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 244 13 شماره
صفحات -
تاریخ انتشار 1969